Pipette suction head - chemical resistance
Release time:
2023-03-23
The accuracy and precision of pipetting requires not only that the pipette tip be matched to the pipette, but also that the tip be suitable for the liquid. When using standard pipette tips (PP) to pipette liquids with low surface tension it is easy to leave a film on the surface of the tip (e.g. liquids containing detergents). The residue of liquid can lead to inaccurate and inconsistent pipetting results, as well as the loss of some expensive samples. The reagents and samples used in many DNA and protein analysis methods often contain detergents.
The accuracy and precision of pipetting requires not only that the pipette tip be matched to the pipette, but also that the tip be suitable for the liquid. When using standard pipette tips (PP) to pipette liquids with low surface tension it is easy to leave a film on the surface of the tip (e.g. liquids containing detergents). The residue of liquid can lead to inaccurate and inconsistent pipetting results, as well as the loss of some expensive samples. The reagents and samples used in many DNA and protein analysis methods often contain detergents.
Materials
Sartorius Optifit standard pipette tip: 1000μ
Sartorius Optifit low adsorption tips: 1000μl
Other manufacturers' low adsorption tips: 1000μl
Sartorius Picus electric pipette: 50-1000μl, aspirate at 4 speeds, drain at 1 speed
Chemical reagents: isopropyl alcohol, acetonitrile, DMF (dimethylformamide), green food dyes
3 low adsorption pipette tips from other manufacturers
96-well microplate spectrophotometer (Biotek)
Absorbance test
In the absorbance test, we use a dye reagent (green food dye dissolved in distilled water) to determine the residual liquid in the pipette tips after draining. The maximum nominal volume of the tip under test is used to aspirate the green reagent. The liquid is then drained directly back into the container. The tips are then rinsed 5 times with distilled water at the maximum volume of the tips. The absorbance of the rinse solution was then measured using a spectrophotometer (405 nm) and the results were compared to the reference reagent. The absorbance of the rinse solution is directly related to the amount of liquid left in the tip.
Chemical resistance test
1000 μl solvent: 20 repeated aspirations of isopropanol, acetonitrile and dimethylformamide using a 1000 μl pipette tip. The tips were rinsed 3 times with distilled water. The effect of this treatment on the performance of the hyposorbent tips was then analyzed using absorbance tests using the stained liquids as reagents. The test was repeated for 6 tips for each solvent. The results of the chemically treated tips were compared to the results of the untreated standard and low adsorption tips.
Chemical Resistance of Low Adsorption Pipette Tips
A variety of techniques have been used to create low adsorption surfaces for pipette tips. The most stable methods produce tips that are completely hydrophobic and do not leach. As shown in Figure 1, there are significant differences in chemical resistance between the low-adsorption tips tested. Some manufacturers' tips show a significant reduction of the hypo-absorption function after treatment with selected solvents. The performance of the Sartorius hyposorbent tips after chemical testing was at the same level as without any chemical treatment, indicating that these tips are inert and leach-free. Autoclaving of Sartorius hyposorbent tips does not affect the performance of the tips either
Hot news
Manufacturer of disposable consumables for biomedical purposes
Changzhou Serbetter Biotech Co., Ltd
Address: No. 26, Huajia Industrial Park, Wujin District, Changzhou City, Jiangsu Province, China
Postal code: 213161
Tel: 0086-519-8988 5218
Fax: 0086-519-89885218
Mobile phone: +8613815085372
WhatsApp: 13961405043
Email: sales@sbt-biotech.com
Copyright © 2023 Changzhou Serbetter Biotech Co., Ltd | IPV6