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Generation, composition and use of lyophilization tubes
The generation of lyophilization tubes edit most of the laboratories in China are currently experimenters make their own strain preservation tubes, which not only increase the work intensity, but also due to various conditions, the strain preservation effect can not always be satisfactory. The creation of strain preservation tubes has made this extremely easy. Freeze storage tube is the common name for the commercial name of strain storage tube.
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Various passages for cell culture flasks
As the name suggests, cell culture bottles are used to culture cells. According to the material, they can be divided into glass and plastic, and the bottom shape can be square, rectangular, triangular, etc. Depending on the bottle volume, there are 5 to 500ml to choose from. In addition, the surface is specially modified to allow better cell wall culture, so that the cell wall area can be divided into 25 to 175cm2.
Detailed steps for cell cryopreservation
Cell cryopreservation is the storage of cells at very low temperatures for thawing and recovery when needed. The following are the steps for general cell lyophilization:
Precautions for pipette tips
Special tips are specially designed tips for special samples or special applications. There are mainly the following categories: the first category is gel tips (also called spot tips): the tip of the tip is either flat or very thin, mainly used for electrophoresis experiments; the second category is wide mouth tips: the tip tip caliber is larger than the general tip, mainly used for removing large molecule solutions such as proteins; the third category is low adsorption tips:
Pipette suction head - chemical resistance
The accuracy and precision of pipetting requires not only that the pipette tip be matched to the pipette, but also that the tip be suitable for the liquid. When using standard pipette tips (PP) to pipette liquids with low surface tension it is easy to leave a film on the surface of the tip (e.g. liquids containing detergents). The residue of liquid can lead to inaccurate and inconsistent pipetting results, as well as the loss of some expensive samples. The reagents and samples used in many DNA and protein analysis methods often contain detergents.
Five pipetting tips to improve your pipetting accuracy
Good pipetting practice, complemented by low adsorption pipette tips, can MAXIMALLY reduce the risks associated with pipetting workflow and improve the accuracy of pipetting and experimental data errors.